Invitrogen™

SuperScript VILO cDNA Synthesis Kit

A first-strand cDNA synthesis kit for two-step real-time PCR workflows.

Read the product overview
Product configuration
Catalog numberConfigurationQuantity
SuperScript™ VILO™ cDNA Synthesis Kit50 reactions

Product overview

A first-strand cDNA synthesis kit for two-step real-time PCR workflows.

SuperScript VILO prepares first-strand cDNA for two-step RT-qPCR. The kit separates its reaction mixture and enzyme blend into two tubes. Its reverse transcriptase is SuperScript III, so this product should be distinguished from the later SuperScript IV VILO formulations.

Selected configuration · 11754050

Format
Kit
No. of Reactions
50 Reactions
Quantity
50 reactions
For Use With (Application)
Real Time PCR (qPCR)
Manufacturer description

ABM product information · English translation

Configurations & pack sizes
Catalog number Unit size Price (GEL)
11754050 is also known as catalog number 11754-050 50 reactions
11754250 is also known as catalog number 11754-250 250 reactions

Specifications

Specifications
Reverse transcriptase: SuperScript™ III
Ribonuclease H activity: -
Downstream application: Quantitative real-time PCR (qPCR)
Reaction format: SuperMix or Master Mix
Sample type: RNA
Final product size(s): 10 kb or less
Optimal reaction temperature: 42° C
Primer type: Random primers
Product line: SuperScript™, VILO™
Product size: 50 reactions
Shipping conditions: Dry ice
Final Product Type
First-Strand cDNA
Format
Kit
No. of Reactions
50 Reactions
Optimal Reaction Temperature
42°C
Quantity
50 reactions
Reaction Format
Master Mix
Reagent Type
Reverse Transcription
Reverse Transcriptase
SuperScript III
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
Size (Final Product)
Up to 10 kb
Starting Material
RNA
Technique
Reverse Transcription
Detection Method
qPCR
For Use With (Application)
Real Time PCR (qPCR)
GC-Rich PCR Performance
High
Reaction Speed
60 min.
Unit Size
Each
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SuperScript VILO cDNA Synthesis Kit
SuperScript VILO cDNA Synthesis Kit

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