Polymerase chain reaction (PCR) method
An overview of polymerase chain reaction and its role in amplifying selected nucleic acid sequences for laboratory analysis.
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An overview of polymerase chain reaction and its role in amplifying selected nucleic acid sequences for laboratory analysis.
PCR amplifies a selected DNA region through repeated temperature cycles using primers and a DNA polymerase. Manufacturer guidance distinguishes the roles of template, primers, enzyme and reaction components. Reagent choice and thermal-cycler requirements depend on the intended application; this listing introduces the method rather than a single product.
- Targeted DNA amplification
- Thermal-cycling method
- Application-specific reagents
Specifications
| # | Equipment: |
|---|---|
| 1. | Class II biological safety cabinet |
| 2. | Refrigerated high-speed benchtop centrifuge |
| 3. | Thermal block |
| 4. | Vortex mixer |
| 5. | Centrifuge |
| 6. | Refrigerator/freezer (×2) |
| 7. | Pipette set: 0–10 μL, 100 μL, 200 μL, 1000 μL, 5000 μL (×2) |
| 8. | Table |
| 9. | Cabinet |
| 10. | Chairs (×3) |
| 11. | Special waste bins (2) |
| 12 | Racks for 1.5–2.0 mL tubes (×4) |
| # | Equipment: |
|---|---|
| 1. | PCR cabinet |
| 2. | Mini centrifuge-vortex mixer |
| 3. | Vortex mixer |
| 4. | Refrigerator/freezer |
| 5. | Pipette set: 0–10 μL, 100 μL, 200 μL, 1000 μL (×2) |
| 6. | Fluorometer |
| 7. | Water purification system |
| 8. | Plate centrifuge |
| 9. | Table |
| 10. | Cabinet |
| 11. | Chairs (×2) |
| 12. | Special waste bins (2) |
| 13. | Applicator |
| 14. | Racks for 1.5–2.0 mL tubes (×4) |
| 15. | 96-well plate racks (×2) |
| # | Equipment |
|---|---|
| 1. | RT-PCR system |
| 2. | Refrigerator |
| 3. | −80°C freezer |
| 4. | Computers (×2) |
| 5. | Tables (×3) |
| 6. | Chairs (×3) |
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